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KMID : 0350519930460031085
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Journal of Catholic Medical College
1993 Volume.46 No. 3 p.1085 ~ p.1097
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Effects of Ceramide on the Cumulative Irritant Skin Reaction Induced by Sodium Lauryl Sulfate Patch Testing
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Abstract
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It is well-known that ceramides, a major component of stratum corneum lipids, may play an important role in water permeability and water retention properties of the skin. Although the precise mechanism of surfactant irritancy is not fuily
understood, it
has been suggested that removal of stratum corneum lipids by surfactants result in skin changes such as scaling, ervthema, and even fissuring.
In this study, several non-invasive measuring methods, including visual scoring, transepidermal water loss(TEWL), electrical conductance and skin replica, were used to evaluate the effect of ceramide in the cumuiative irritant skin reactions
induced by
sodium lauryl sulfate(SLS), a simple anionic surfactant. A 0.5 % SLS solution was spplied on three volar sites of both forearms(B, C & D) in 10 volunteers for 45 minutes each at a three hour interval twice a day and distilled water(D/W) for
control
group from Monday to Friday for 2 weeks. About 10 minutes after removal of each SLS application, a standard amount of ceramide and its vehicle(0.05 mg) was topically applied to SLS-induced irritant skin reaction sites9D & C), respectively. Each
measurement was made in the morning before any application was made on days 1, 5, 8, 12 and 15.
@ES The results were as follows ;
@EN 1. The values of mean visual score measured at group C and D were significantly lower than that measured at B group9P<0.05), but the difference between group C and group D was not significant.
2. The values of transepidermal water loss(FEWL) measured at group B, C and D were significantly increased as compared with that at the control group(A) with time(P<0.01). The increase in TEWL values group C and D was significantly lower than
that
at
group B(P<0.01). Furthermore, an increase in TEWL at group D was significantly lower than that at group C(P<0.05).
3. The values of electrical conductance measured at group B, C and D were significantly decreased as compared with that at the control group(A) with time(P<0.01). The decrease in values of electrical conductance measured at group C and D was
less
than
that at group B(P<0.05), but the difference between group C and D was not significant.
4. The mean visual score was significantly correlated with TEWL(r=0.93, P<0.05) or electrical conductance(r=-0.92, P<0.05). A strong negative correlation between TEWL and electrical conductanece measured at group B, C and D was found(r=-0.95,
P<0.05).
5. The Scanning eletron microscopic findings of skin replicas showed a decrease in surface roughness and ridge height, and a slight increase in distance between ridges at SLS-induced skin lesions after two weeks of SLS applications.
These results suggest that topical applicantion of ceramides may improve the disruption of permeability barrier rather than that of the water holding capacity of the stratum corneum induced by repeated exposure to sufactants.
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KEYWORD
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